ZIKV-IN-3

Category	Virus Protease
CAS	947699-46-9
Description	ZIKV-IN-3 (compound 5a), an andrographolide derivatives, is a potent ZIKV NS5 methyl transferase (MTase) inhibitor with an IC50 value of 18.34 μM. ZIKV-IN-3 inhibits ZIKV replication and infection. ZIKV-IN-3 can be used in research of Zika virus (ZIKV).

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Molecular Weight	587.75
Molecular Formula	C39H41NO4
Purity	≥98% (HPLC)
Solubility	In vitro: 10 mM in DMSO
Appearance	Solid powder
Storage	Store at -20°C
In Vitro	ZIKV-IN-2 (compound 5a; 0.08-50 μM) inhibits ZIKV-induced plaque formation with an EC50 value of 0.76 μM and has anti-ZIKV effect with EC50 values of 0.48, 0.37, and 0.45 μM for Vero, Huh7 and A549 cells, respectively. ZIKV-IN-2 (0.08-50 μM; 48 h) inhibits the expression of ZIKV E protein with an EC50 value of 0.38 μM and inhibits ZIKV replication and infection. ZIKV-IN-2 (0.08-50 μM; 96 h) has low cytotoxicity in Vero, Huh7 and A549 cells.Vero, Huh7 and A549 cells. Cell Viability Assay Cell Line: Vero, Huh7 and A549 cells Concentration: 0.08, 0.4, 2, 10, and 50 μM Incubation Time: 96 hours Result: Exhibited low cytotoxicities to Vero cells, Huh7 cells and A549 cells with CC50>200 μM to all three cell lines. Western Blot Analysis Cell Line: Vero cells Concentration: 0.08, 0.4, 2, 10, and 50 μM Incubation Time: 48 hours Result: Inhibited the expression of ZIKV E protein in a dose-dependent manner.
Target	IC50: 18.34 μM (MTase)