NH125

Category	Hepatitis C Virus (HCV)
CAS	278603-08-0
Description	NH125 is a potent and selective eEF-2 kinase inhibitor with an IC50 of 60 nM, and also a potent histidine kinase inhibitor. It shows >125-fold selectivity over PKC, PKA, and CaMKII.

Quotation Now

Product Information

Synonyms	3-Benzyl-1-hexadecyl-2-methyl-1H-imidazol-3-ium Iodide; 1-hexadecyl-2-methyl-3-(phenylmethyl)-1H-Imidazolium iodide; NSC717205; 1-Benzyl-3-cetyl-2-methylimidazolium iodide; 1H-Imidazolium, 1-hexadecyl-2-methyl-3-(phenylmethyl)-, iodide (1:1)
IUPAC Name	1-benzyl-3-hexadecyl-2-methylimidazol-1-ium;iodide
Molecular Weight	524.56
Molecular Formula	C27H45IN2
Canonical SMILES	CCCCCCCCCCCCCCCCN1C=C[N+](=C1C)CC2=CC=CC=C2.[I-]
InChI	InChI=1S/C27H45N2.HI/c1-3-4-5-6-7-8-9-10-11-12-13-14-15-19-22-28-23-24-29(26(28)2)25-27-20-17-16-18-21-27;/h16-18,20-21,23-24H,3-15,19,22,25H2,1-2H3;1H/q+1;/p-1
InChIKey	RVWOHCBHAGBLLT-UHFFFAOYSA-M
Melting Point	88.2-94.9°C
Purity	>98%
Solubility	Soluble in DMSO
Appearance	White to Off-white Solid
Storage	Store at 2-8°C, sealed storage, away from moisture
Complexity	364
Exact Mass	524.26275
In Vitro	NH125 inhibits eEF-2 kinase activity (IC50 = 60 nM) in vitro, blocks the phosphorylation of eEF-2 in intact cells, and shows relative selectivity over other protein kinases: protein kinase C (IC50 = 7.5 μM), protein kinase A (IC50 = 80 μM), and calmodulin-dependent kinase II (IC50 > 100 μM). NH125 decreases the viability of 10 cancer cell lines with IC50s ranging from 0.7 to 4.7 μM. Forced overexpression of eEF-2 kinase in a glioma cell line produces 10-fold resistance to NH125. In conclusion, these results suggest that identification of potent inhibitors of eEF-2 kinase may lead to the development of new types of anticancer drugs. Anticancer effect of NH125 is not mediated through inhibition of eEF2K. Inhibition of cell growth correlates with induction of peEF2.
PSA	8.81000
Target	CaMK III: 60 nM (IC50)