NGI 1

Category	Virus Protease
CAS	790702-57-7
Description	NGI 1 is a cell-permeable oligosaccharyltransferase (OST) inhibitor. It exhibits a potent activity against flaviviruses via blocking viral RNA replication. It also selectively arrests proliferation of non-small-cell lung cancer cells that are dependent on EGFR or FGFR for survival.

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Synonyms	NGI-1; NGI1; ML414; ML 414; ML-414; 5-[(Dimethylamino)sulfonyl]-N-(5-methyl-2-thiazolyl)-2-(1-pyrrolidinyl)benzamide
IUPAC Name	5-(dimethylsulfamoyl)-N-(5-methyl-1,3-thiazol-2-yl)-2-pyrrolidin-1-ylbenzamide
Molecular Weight	394.51
Molecular Formula	C17H22N4O3S2
Canonical SMILES	CC1=CN=C(S1)NC(=O)C2=C(C=CC(=C2)S(=O)(=O)N(C)C)N3CCCC3
InChI	InChI=1S/C17H22N4O3S2/c1-12-11-18-17(25-12)19-16(22)14-10-13(26(23,24)20(2)3)6-7-15(14)21-8-4-5-9-21/h6-7,10-11H,4-5,8-9H2,1-3H3,(H,18,19,22)
InChIKey	QPKGRLIYJGBKJL-UHFFFAOYSA-N
Purity	≥98% by HPLC
Density	1.4±0.1 g/cm3
Solubility	In vitro: 10 mM in DMSO
Appearance	White to off-white (Solid)
Storage	Powder: -20°C: 3 years 4°C: 2 years In solvent: -80°C: 6 months -20°C: 1 month
Complexity	602
Exact Mass	394.11333292
Index Of Refraction	1.634
In Vitro	NGI-1 inhibits the glycosylation of LASV GP mediated by STT3A-OST (in STT3B- and MAGT1-TUSC3- cells) or STT3B-OST (in STT3A- cells) and impaires its proteolytic cleavage in a dose-dependent manner. NGI-1 blocks EGFR N-linked glycosylation in lung adenocarcinoma cells as assessed. In controls EGFR is biotinylated, consistent with its plasma membrane expression, but in NGI-1 treated cells the EGFR is predominantly found in the non-biotinylated intracellular fraction suggesting a change in cellular localization.
Target	Virus Protease
XLogP3-AA	2.3